Search, design, and evaluate CRISPR guides in one workspace.

Explore genome targets, generate candidate spacers, and assess multiplex guide compatibility using trained genomic models.

Research prototype Predictions support computational exploration and are not experimental validation of editing, cleavage, safety, or efficacy.

Genome target search

Find exact and nearby guide matches

Species model Cas system PAM pattern

Spacer sequenceEnter one strict 20-nt spacer per line.

Advanced search settings

Results per spacer Maximum mismatches Search depth Model partitions limit Model version

Require PAM matchShow nearest candidates

Candidate guide design

Generate and screen candidate spacers

Design

Species model Cas system PAM pattern

Target type Preferred region

TargetEnter a gene, identifier, genomic interval, or DNA sequence.

Advanced design settings

Return top candidates Candidates to screen Minimum GC Maximum GC Off-target mismatches Model partitions limit Model version

Multiplex panel analysis

Evaluate guides individually and as a panel

Multiplex

Species model Cas system PAM pattern

Guide panelOne guide per line. Optional labels may be separated by a tab, comma, or vertical bar.

Advanced multiplex settings

Recommended panel size Top panel combinations Hits per guide Maximum mismatches Search depth Minimum GC Maximum GC Model partitions limit Model version

Require PAM-compatible hits

Analysis output

Results

✂No analysis yet

Choose a mode and submit an analysis to view results.

guntingAIA ChordexBio research prototype powered by Covary genomic intelligence.

Computational predictions require independent experimental and safety validation.